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The thiols, organic compounds characterized by having in its molecule a sulphydryl group (-SH), represent a significant component of quality of plasma antioxidant barrier deployed against free radicals. In fact, they are able to oppose the propagation phase of peroxidation processes, for their ability to inactivate both the alkoxyl radical which peroxyl ones. These include cysteine (isolated or incorporated into peptides, such as glutathione, or in real proteins) and lipoic acid.
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The -SHp Test is based on the ability of thiols to develop a colored complex can be determined photometrically when they react with a particular acid (DTNB), by the method initially proposed by Ellman in 1959 and then adapted by Carratelli. The "title" of thiols is directly proportional to the intensity of color instrumentally detected.
The range found in normal subjects is 450-650 mmol/L. A reduction in test values below this range indicates a condition of oxidative stress by lowering of antioxidant defenses. In this case, will the physician should determine, through the inclusion of test results in the clinical picture of each specific patient, what strategy should be put in place in order to bring back to normal any outliers.
The test is particularly useful in detecting conditions of a shortage of thiolic antioxidants (lipoic acid, glutathione, cysteine) and monitoring efficacy of formulations of active ingredients containing SH-groups (e.g. N-acetylcysteine, proteins from whey, lipoic acid, etc.)..
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