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d-ROMs test: colorimetric determination of reactive oxygen metabolites Hydroperoxides, the “markers” and “amplifiers” of tissue damage generated by peroxidation of lipids, amino acids, proteins, and nucleic acids, are relatively stable and maintain in biological fluids a good oxidant capacity. Therefore, in this test, hydroperoxides (a class of ROMs), after reacted with an opportunely buffered chromogen, develop a coloured derivative, which is photometrically detected. The concentration of ROMs, that directly parallels with colour intensity, is expressed as Carratelli Units (1 CARR U = 0.08 mg% hydrogen peroxide). The range in healthy peoples is 250-300 U CARR. Increased values directly correlate to increased levels of oxidative stress. The d-ROMs test is very useful in clinical practice. |  FREE |
FREE (i. e. Free Radicals Elective Evaluator) is an integrated analytical system consisting of a photometer and a minicentrifuge,that is able to perform any chemical analyses based on the principles of spectrophotometry (i.e. cholesterol, blood glucose etc.) and, particularly, the whole panel of oxidative stress tests made by Diacron (d-ROMs test, OXY-adsorbent test and -SHp test). FREE, is a special device to best investigate the oxidative stress and therefore it is specifically designed for researchers and laboratory analysts. A learning course for using it is required. | |
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Oxy- Adsorbent test: determination of plasma antioxidant potential In the plasma a number of compounds (e. g. carotenoids, ascorbate, vitamin E, bilirubin, uric acid, etc) are able to “adsorb” the oxidant “potential” of reactive species. Therefore, any injury to “the plasma barrier to oxidation” can result in oxidative tissue damage. In this context, OXY-Adsorbent test evaluates the ability of plasma to oppose the massive oxidant action of an excess of hypochlorous acid in water solution. This goal is hit by assessing photometrically the residual unreacted radicals of the acid. Normally, 1 mL of human plasma is able to adsorb at least 350 mmoles of hypochlorous acid. Decreased values directly correlate to the injury severity of “plasma barrier to oxidation”. In fact, when the “excess” of radicals of hypochlorous acid after massive oxidation is high, the plasma barrier is reduced, and vice versa. |  FRAS 3 |
FRAS 3 (i. e. Free Radical Analytical System) is an
integrated analytical system that by the d-ROMs test (i. e. test to
evaluate Reactive Oxygen Metabolites, ROMs) performed on a capillary
blood sample is able to assess the oxidative stress. The oxidative stress is a kind of chemical stress that results from the presence in a living organism of a free radicals excess. FRAS 3 is a device specifically designed for physicians and specialists. A learning course is not required. |
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-SHp test: determination of plasma thiols Protein thiols are a significant component of the “plasma barrier to oxidation”. In fact, thiol groups of serum proteins are able to oppose the propagation step of peroxidative processes by inactivating either alkoxyl or hydroxyl radicals. This test is based on the ability of -SH groups to develop a coloured complex when reacted with 5,5-dithiobys-2-nitrobenzoyc acid (DTNB). The “titre” of thiols directly parallels with colour intensity. The range in healthy peoples is 450-650 mmoles -SH/L. Decreased values directly correlate to a lowered efficacy of thiols antioxidant barrier. | |||
